Epithalon (Epitalon) Dosing Protocol: 20 mg Vial — Two-Course Annual Supply Guide
Quickstart highlights
The 20 mg vial allows two complete Epithalon 10-day intensive courses using partial reconstitution.
- Concentration: 2 mg/mL per reconstitution (reconstitute 5 mL at a time, = 10 mg per reconstitution).
- One 20 mg vial = 2 complete 10-day courses at 10 mg/day.
- Partial reconstitution: reconstitute 5 mL for Course 1; remaining 10 mg dry powder stores safely for months.
- Standard protocol: twice-yearly courses (spring + autumn) at 10 mg/day x 10 days.
- Inject in the evening; use 3 mL syringe for 5 mL injection volume.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
| Week | Dose (µg) | Units | Frequency | Notes |
|---|---|---|---|---|
| — | 10000 | 500 | Once daily x 10 days (evening) | 10 mg — 500 units; reconstitute first 5 mL (half the vial) for course 1 |
| — | 10000 | 500 | Once daily x 10 days (evening) | Reconstitute remaining 5 mL for course 2 — the dry peptide retains full potency until second reconstitution |
Your free Epithalon (Epitalon) tracker
- Dose reminders
- Injection logging
- Cycle progress
Reconstitution steps
- For Course 1: Draw 5 mL bacteriostatic water; inject into the 20 mg vial slowly down the wall.
- This reconstitutes 10 mg at 2 mg/mL — sufficient for a 10-day course at 10 mg/day.
- Store reconstituted vial at 2–8 °C; use within 28 days.
- For Course 2 (months later): The remaining dry peptide powder remains in the vial — draw another 5 mL bac water and reconstitute the second 10 mg portion.
Supplies needed
per_course-week plan
- 0.5 vials
- 10 syringes
- 5 mL bac water
- 10 alcohol swabs
annual_two_courses-week plan
- 1 vial
- 20 syringes
- 10 mL bac water
- 20 alcohol swabs
Protocol overview & cycle notes
Execute two semi-annual 10-day Epithalon intensive courses (spring and autumn) from a single 20 mg vial using partial reconstitution, for cost-efficient long-term telomere maintenance and anti-aging support.
Off-cycle: 4–6 months between each 10-day intensive course; 2 courses per year from one 20 mg vial.
Storage & handling
Injection & tracking tips
- Perform the second reconstitution of the remaining dry powder no earlier than 4 months after the first course, to respect the 28-day use window of the second reconstitution.
- Label the vial with Course 1 reconstitution date; mark when Course 2 is planned.
- The two-course-per-year schedule (spring + autumn) aligns with traditional Russian bioregulator protocols.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
- Test telomere length before the first course and 6 months post-second course for long-term assessment.
- Track melatonin quality indicators (sleep latency, depth) around each course.
- Monitor IGF-1 and DHEA-S annually as longevity biomarkers.
How this works & references
Sources
- Peptides of pineal gland and thymus prolong human life. Khavinson VKh et al. — Neuro Endocrinol Lett — 2003
- Source: Anisimov VN et al. — Long-term mortality in Epithalon-treated cohort. Biogerontology. 2011
- Antioxidant properties of geroprotective peptides of the pineal gland. Kozina LS et al. — Arch Gerontol Geriatr — 2007
Frequently asked questions
Is it safe to partially reconstitute a lyophilized peptide vial?
Should the 20 mg vial be stored differently before the second reconstitution?
Do I need two separate vials for a twice-yearly protocol?
Is there a lower-dose option for Epithalon?
What evidence supports twice-yearly versus once-yearly Epithalon courses?
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View protocolThis content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.