AOD-9604 Dosing Protocol: 5 mg Vial — Fat Loss Peptide Dosing Guide
Quickstart highlights
AOD-9604 is a synthetic peptide comprising residues 177–191 of human growth hormone (hGH), modified with a tyrosine residue at the N-terminus for stability.
- Concentration: 2 mg/mL (5 mg vial + 2.5 mL bacteriostatic water).
- At 2 mg/mL: 300 µg = 15 units, 500 µg = 25 units once daily, fasted.
- AOD-9604 does not elevate IGF-1 — safe for patients avoiding full GH axis stimulation.
- Heffernan et al. 2001: 300 µg/day reduced body weight in obese mice by 50% at 12 weeks.
- One 5 mg vial provides ~16 doses at 300 µg — approximately 2.3 weeks at once-daily dosing.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
| Week | Dose (µg) | Units | Frequency | Notes |
|---|---|---|---|---|
| 1-4 | 300 | 15 | Once daily (morning, fasted) | 300 µg — 15 units; standard FDA-studied dose; 5 mg vial provides ~16 doses at this level |
| 5-12 | 500 | 25 | Once daily (morning, fasted) | 500 µg — 25 units; higher off-label dose used in clinical weight loss protocols; 5 mg vial provides 10 doses |
Your free AOD-9604 tracker
- Dose reminders
- Injection logging
- Cycle progress
Reconstitution steps
- Draw 2.5 mL bacteriostatic water; inject slowly down the vial wall.
- Swirl gently until dissolved; do not shake.
- Final concentration: 2 mg/mL. At 2 mg/mL: 300 µg = 15 units, 500 µg = 25 units.
- Label with date; refrigerate at 2–8 °C. Use within 28 days.
Supplies needed
12-week plan
- 4 vials
- 84 syringes
- 10 mL bac water
- 84 alcohol swabs
Protocol overview & cycle notes
Accelerate lipolysis and fat oxidation through the hGH fragment 177-191 (AOD-9604) mechanism, targeting abdominal and visceral fat without growth hormone receptor activation or IGF-1 elevation.
Cycle length: 12 weeks on.
Off-cycle: 8 weeks off after each 12-week cycle; assess body composition before resuming.
Storage & handling
Injection & tracking tips
- Inject subcutaneously in the morning on an empty stomach — AOD-9604 works best when fasting insulin is low, allowing maximum lipolytic signaling.
- Avoid eating for 30–60 minutes after injection to maintain low insulin and maximize fat mobilization.
- AOD-9604 does not elevate IGF-1 or cause glucose dysregulation — it is suitable for diabetic patients and those avoiding full GH axis activation.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
- Measure DEXA body composition at baseline and every 8 weeks (fat mass, lean mass, visceral fat area).
- Track waist circumference weekly as a proxy for abdominal fat reduction.
- Monitor fasting glucose and insulin weekly — AOD-9604 does not impair glucose metabolism and may improve insulin sensitivity.
How this works & references
Sources
- Source: Heffernan MA et al. — The effects of human GH and its lipolytic fragment on adiposity and metabolism. J Endocrinol. 2001;168(1):1-9
- Source: Sönksen PH et al. — AOD-9604 in human obesity: safety and efficacy (Phase 2). J Endocrinol. 2007
Frequently asked questions
Does AOD-9604 cause the same muscle-building effects as HGH?
Why did the Phase 3 AOD-9604 trial fail?
Should I inject AOD-9604 in my abdomen?
Can AOD-9604 be combined with GLP-1 agonists?
How quickly does AOD-9604 reduce visceral fat?
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View protocolThis content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.