TB-500 Dosing Protocol: 5 mg Vial — Loading & Maintenance Schedule
Quickstart highlights
TB-500 is a synthetic peptide corresponding to the active fragment (Ac-SDKP, 17 amino acids from the C-terminus) of Thymosin Beta-4 — a 43-amino acid actin-sequestering protein ubiquitous in human tissue.
- Concentration: 2 mg/mL (5 mg vial + 2.5 mL bacteriostatic water).
- At 2 mg/mL: 2 mg = 100 units on a U-100 syringe.
- Loading phase: 2 mg 2x weekly for 4–6 weeks; maintenance: 2 mg every 2 weeks.
- TB-500 acts systemically — inject in abdomen, not at injury site.
- One 5 mg vial provides ~2.5 loading doses (2 mg each); plan 4 vials for a 6-week loading phase.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
| Week | Dose (µg) | Units | Frequency | Notes |
|---|---|---|---|---|
| 1-6 | 2000 | 100 | Twice weekly | 2 mg 2x weekly — 100 units each; loading phase; one 5 mg vial provides ~2.5 injections |
| 7+ | 2000 | 100 | Every 2 weeks | 2 mg every 2 weeks — maintenance phase; one 5 mg vial provides 2 maintenance doses |
Gradual approach
| Week | Dose (µg) | Units | Notes |
|---|---|---|---|
| 1-8 | 1000 | 50 | 1 mg 2x weekly — conservative loading; suitable for sensitive patients or first TB-500 cycle |
| 9-12 | 2000 | 100 | Advance to 2 mg 2x weekly if 1 mg well-tolerated |
Your free TB-500 tracker
- Dose reminders
- Injection logging
- Cycle progress
Reconstitution steps
- Draw 2.5 mL bacteriostatic water; inject slowly down the vial wall.
- Swirl gently until lyophilized powder dissolves; do not shake — TB-500 is susceptible to degradation from mechanical agitation.
- Final concentration: 2 mg/mL. At 2 mg/mL: 2 mg = 100 units on a U-100 syringe.
- Label with date; refrigerate at 2–8 °C. Use within 28 days.
Supplies needed
8-week plan
- 4 vials
- 16 syringes
- 10 mL bac water
- 16 alcohol swabs
12-week plan
- 7 vials
- 24 syringes
- 18 mL bac water
- 24 alcohol swabs
Protocol overview & cycle notes
Promote systemic tissue repair, reduce inflammation, and accelerate injury recovery through the TB-500 loading-then-maintenance protocol, leveraging Thymosin Beta-4's unique actin-sequestering and stem cell-mobilizing properties.
Cycle length: 12 weeks on.
Off-cycle: 4–8 weeks off after 12-week cycle.
Storage & handling
Injection & tracking tips
- TB-500 has systemic effects unlike BPC-157 — injection site does not need to be near the injury; abdomen is preferred for reliable subcutaneous absorption.
- Do not shake the reconstituted vial; TB-500 (as a fragment of the 43-amino acid Thymosin Beta-4) is more prone to mechanical degradation than smaller peptides.
- For combination with BPC-157: use separate syringes and separate injection sites; TB-500 and BPC-157 can be used on the same days.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
- Track injury pain (0–10 VAS) and range of motion weekly during loading phase.
- Log systemic effects: fatigue levels, exercise recovery, and any soft tissue inflammation indicators.
- Measure inflammatory markers (CRP, ESR) at baseline and after 6 weeks if clinically indicated.
How this works & references
Frequently asked questions
What is the difference between TB-500 and Thymosin Beta-4?
Should I inject TB-500 near the injury, like BPC-157?
How long does the loading phase need to be?
Can TB-500 and BPC-157 be used simultaneously?
Is TB-500 WADA-prohibited?
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View protocolThis content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.