Semaglutide 5 mg Vial Dosing Protocol
Quickstart highlights
Semaglutide is a GLP-1 receptor agonist that enhances insulin secretion and suppresses appetite.
- Concentration: 3.33 mg/mL (5 mg vial + 1.5 mL bac water).
- Dose range: 300 µg (Week 1–2) up to 1,000 µg (maintenance) once weekly.
- At 3.33 mg/mL, 1 unit ≈ 33.3 µg; use a U-100 insulin syringe.
- Refrigerate reconstituted solution; use within 28–56 days.
Dosing table
For educational reference only. Your prescribing provider may adjust doses based on your clinical profile and response.
| Week | Dose (µg) | Units | Frequency | Notes |
|---|---|---|---|---|
| 1-2 | 300 | 9 | Once weekly | Starting dose — use a 30-unit U-100 insulin syringe |
| 3-4 | 600 | 18 | Once weekly | Pause if GI side effects persist |
| 5-6 | 1000 | 30 | Once weekly | Maintenance — switch to 100-unit syringe for easier measurement |
Gradual approach
| Week | Dose (µg) | Units | Notes |
|---|---|---|---|
| 1-4 | 250 | 7.5 | Slower ramp — use 30-unit syringe |
| 5-8 | 500 | 15 | Increase only if tolerating well |
| 9+ | 1000 | 30 | Maintenance — switch to 100-unit syringe |
Your free Semaglutide 5 mg Vial tracker
- Dose reminders
- Injection logging
- Cycle progress
Reconstitution steps
- Use 1.5 mL bacteriostatic water; inject slowly down the vial wall to yield 3.33 mg/mL.
- Gently swirl until fully dissolved; do not shake.
- Label the vial with reconstitution date; refrigerate 2–8 °C.
Supplies needed
8-week plan
- 1 vial
- 8 syringes
- 2 mL bac water
- 8 alcohol swabs
12-week plan
- 2 vials
- 12 syringes
- 3 mL bac water
- 12 alcohol swabs
Protocol overview & cycle notes
Support weight management with a GLP-1 agonist titration starting from a 5 mg compounded vial. Targets 1 mg weekly maintenance after 4–6 weeks.
Cycle length: 12 weeks on.
Off-cycle: 8 weeks off between cycles; monitor mood and energy during off-cycle.
Storage & handling
Injection & tracking tips
- Rotate injection sites (abdomen, thigh, upper arm) to reduce irritation.
- Use a 30-unit U-100 insulin syringe for early low doses (9 units); switch to a 100-unit U-100 syringe once you exceed 20 units.
Tracking
Logging helps you and your provider spot patterns and adjust dose or timing.
- Log injection date, dose (units), and injection site.
- Track appetite, energy, and any side effects weekly.
How this works & references
Sources
- Once-Weekly Semaglutide in Adults with Overweight or Obesity. Wilding JPH et al. — N Engl J Med — 2021
- FDA – Wegovy (semaglutide) prescribing information
- Clinical Pharmacokinetics of Semaglutide: A Systematic Review. Yang XD et al. — Drug Des Devel Ther — 2024
- Pharmacokinetics, Safety and Tolerability of Once-Weekly Subcutaneous Semaglutide in Healthy Chinese Subjects: A Double-Blind, Phase 1, Randomized Controlled Trial. Shi A et al. — Adv Ther — 2021
Frequently asked questions
How much bac water do I add to a 5 mg semaglutide vial?
How many doses are in a 5 mg vial?
What syringes do I use for a 5 mg vial?
Should I choose a 5 mg or 10 mg vial?
Related protocols
KLOW Blend Dosing Protocol — BPC-157, TB-500, KPV & GHK-Cu Guide
KLOW peptide blend (BPC-157 + TB-500 + KPV + GHK-Cu) dosing guide for tissue repair, anti-inflammatory support, and extracellular matrix remodeling — the GLOW blend plus KPV.
View protocolGLOW Blend Dosing Protocol — BPC-157, TB-500 & GHK-Cu Skin & Repair Guide
GLOW peptide blend (BPC-157 + TB-500 + GHK-Cu) dosing guide for tissue repair, angiogenesis, and collagen and connective-tissue remodeling.
View protocolVilon Dosing Protocol: 20 mg Vial — Dipeptide Immune & Longevity Bioregulator Guide
Vilon (Lys-Glu dipeptide) Russian immune and longevity bioregulator dosing guide — the simplest Khavinson bioregulator for systemic immune normalization and longevity support.
View protocolVesugen Dosing Protocol: 20 mg Vial — Vascular Bioregulator & Cardiovascular Guide
Vesugen (Lys-Glu-Asp tetrapeptide) Russian vascular bioregulator dosing guide — endothelial cell gene normalization for arterial health, blood pressure regulation, and cardiovascular aging.
View protocolThis content is for informational purposes only and does not constitute medical advice. Consult a licensed healthcare provider before starting any peptide therapy. Dosing and protocols may vary by formulation and prescriber.